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Flowchart of the serological tests conducted to detect recent or past ZIKV and DENV infections in serum samples of all postpartum women participants ( n = 601). The samples were screened using an ELISA to detect specific IgG antibodies with all positive results confirmed by the CPE‐VNT technique to detect neutralizing antibodies. Discordant samples were further analyzed using the PRNT assay. Once true positive samples were identified they were subsequently tested for the presence of anti‐ZIKV and anti‐DENV specific IgM antibodies.

Journal: Journal of Medical Virology

Article Title: Seroprevalence and Placental Transfer of Zika and Dengue Virus Antibodies in Postpartum Women in Southeast Brazil

doi: 10.1002/jmv.70384

Figure Lengend Snippet: Flowchart of the serological tests conducted to detect recent or past ZIKV and DENV infections in serum samples of all postpartum women participants ( n = 601). The samples were screened using an ELISA to detect specific IgG antibodies with all positive results confirmed by the CPE‐VNT technique to detect neutralizing antibodies. Discordant samples were further analyzed using the PRNT assay. Once true positive samples were identified they were subsequently tested for the presence of anti‐ZIKV and anti‐DENV specific IgM antibodies.

Article Snippet: For the detection of anti‐ZIKV and anti‐DENV IgM antibodies, the anti‐Zika virus IgM μ‐capture ELISA kit (#ab213327, Abcam, Cambridge, USA) and the Panbio Dengue IgM Capture ELISA kit (#01PE20, Abbott, Chicago, IL, USA) were used, respectively, following the manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Plaque Reduction Neutralization Test